RT-PCR is a technique as useful as it is ambiguously named. In this case, I’m referring to real-time PCR, also known as quantitative PCR (qPCR), but not to be confused with reverse transcriptase PCR ...

Are you new to quantitative (or “real-time”) PCR? Maybe you’re just looking to brush up on the basics of designing and analyzing qPCR experiments. Perhaps your reactions aren’t working. Again.

Polymerase chain reaction (PCR) is a fundamental molecular biology tool that scientists use to amplify and analyze genetic material, such as DNA and RNA. PCR involves the enzymatic amplification of ...